It comes after Secretary of State Antony Blinken said the US had evidence that Russia had made plans for a "large scale" attack on Ukraine.
But he added it was unclear if Mr Putin had made a final decision to invade.
Russia has denied any such intention, and accused Ukraine of executing its own troop build-up.
In a statement released on Saturday evening, White House Press Secretary Jen Psaki said that Mr Biden will "underscore US concerns with Russian military activities on the border with Ukraine and reaffirm the United States' support for the sovereignty and territorial integrity of Ukraine" during his call with the Russian president.
Ukraine says Russia has deployed armoured vehicles, electronic warfare systems and 94,000 troops along their shared border.
It is the largest massing of Russian forces on its borders since it annexed Crimea from Ukraine in 2014.