The booing happened during an awards ceremony after the men's final, when a tennis official referred to global vaccine efforts as a sign of optimism.
It comes just as Australia begins its national inoculation programme.
Deputy Prime Minister Michael McCormack called the crowd's behaviour "disgusting".
"I don't like booing at any event, and certainly any sporting event," he told reporters in Canberra on Monday.
"This vaccine is going to get our country back to some sort of pre-Covid normality," he said.