Just over a week before the opening ceremony, the spreading infections highlight the risks of staging the world's biggest sports event during a pandemic even without spectators in sports venues.
Seven staff at the hotel in Hamamatsu city, southwest of Tokyo, had tested positive for the coronavirus, a city official said.
But a 31-strong Brazilian Olympic delegation, which includes judo athletes, are in a "bubble" in the hotel and separated from other guests and have not been infected.