The firm, which employs 1.5 million people worldwide, said the cuts would fall mainly in areas including cloud computing and advertising.
It did not say which countries would be affected but said the positions would be closed in the next few weeks.
Boss Andy Jassy said it was a "difficult decision" but it would be best for the company in the long term.
The firm already axed 18,000 jobs in January.
Mr Jassy said that in recent years, most areas of Amazon's business had been adding roles.